Saratov Airlines Flight 703 went down on Sunday near Ramenskoye town, about 40km from Moscow's second-largest airport.
Maxim Sololov, Russia's transportation minister, confirmed there were no survivors.
The Antonov An-148 aircraft had 65 passengers and six crew members on board when it went down.
The plane was heading from the Russian capital to Orsk city, about 1,500km away, near the border with Kazakhstan.
It was not immediately clear what caused the crash.
Witnesses said they saw a burning plane falling out of the sky.